A common problem with Windows is that when you plug in a USB drive it may acquire a new drive letter. Usually this is no problem, but if you use the drive with a program that wants the data to be on the same drive each time it is inserted, you need to force Windows to use the same drive letter. Doing so is not hard. Here I will illustrate using Windows 7.
Click START and type in diskmgmt.msc in the search box. Press enter to run the program.